RESEARCH PAPER
Analysis of selected virulence factors of bacteria and yeast isolated from work environments in composting plants, tenneries, museums
1
Instytut Technologii Fermentacji i Mikrobiologii, Politechnika Łódzka Dyrektor Instytutu: dr hab. B. Gutarowska prof. nadzw.
Corresponding author
Justyna Skóra
Instytut Technologii Fermentacji i Mikrobiologii Politechnika Łódzka ul. Wólczańska 171/173, 90-924 Łódź tel. (42) 631-34-70
Instytut Technologii Fermentacji i Mikrobiologii Politechnika Łódzka ul. Wólczańska 171/173, 90-924 Łódź tel. (42) 631-34-70
Med Srod. 2014;17(3):52-61
KEYWORDS
ABSTRACT
Introduction:
The aim of the study was to evaluate the production of selected virulence factors by potential pathogenic microorganisms isolated in workplaces. The influence of technical material present at the workplaces on strains virulence was also determined.
Material and Methods:
11 bacteria and yeast strains isolated from the air (impact method) or surface (RODAC method) in work environments. Identification was performed by API tests and molecular methods. The selected factors were analyzed: production of polysaccharide capsules, proteinase, gelatinase, lipase, coagulase, deoxyribonuclease, enterotoxins and hemolytic abilities. Apart from standard microbiological media, minerals media with addition of cellulose, wet blue leather, compost extract were used.
Results:
8 from 11 tested strains produced hemolysis, including 4 bacterial strains (Bacillus cereus two strains, Bacillus subtilis, Staphylococcus haemolyticus) – β-hemolysis. Polysaccharide capsules were detected for yeast Cryptococcus albidus. Bacteria, mainly from the genus Bacillus, produced protease and gelatinase. Moreover, B. cereus strains from composting plants and tanneries produced enterotoxins (NHE and HBL). The presence of leather or compost in the medium can stimulate or inhibit toxin production, depending on the bacteria species and toxin type. S. haemolyticus from the museum produced lipase and deoxyribonuclease. It was found that Corynebacterium lubricantis and Candida parapsilosis did not produce any of the tested virulence factors
Conclusions:
In the work environment in composting, tanneries, museums with high frequency (56–100%) there are potentially pathogenic organisms: Bacillus cereus, B. pumilus, B. subtilis, Cryptococcus albidus, Pseudomonas vancouverensis, Staphylococcus heamolyticus able to produce virulence factors (polysaccharide capsules, proteinase, gelatinase, lipase, coagulate, deoxyribonuclease, enterotoxins, haemolysins).
The aim of the study was to evaluate the production of selected virulence factors by potential pathogenic microorganisms isolated in workplaces. The influence of technical material present at the workplaces on strains virulence was also determined.
Material and Methods:
11 bacteria and yeast strains isolated from the air (impact method) or surface (RODAC method) in work environments. Identification was performed by API tests and molecular methods. The selected factors were analyzed: production of polysaccharide capsules, proteinase, gelatinase, lipase, coagulase, deoxyribonuclease, enterotoxins and hemolytic abilities. Apart from standard microbiological media, minerals media with addition of cellulose, wet blue leather, compost extract were used.
Results:
8 from 11 tested strains produced hemolysis, including 4 bacterial strains (Bacillus cereus two strains, Bacillus subtilis, Staphylococcus haemolyticus) – β-hemolysis. Polysaccharide capsules were detected for yeast Cryptococcus albidus. Bacteria, mainly from the genus Bacillus, produced protease and gelatinase. Moreover, B. cereus strains from composting plants and tanneries produced enterotoxins (NHE and HBL). The presence of leather or compost in the medium can stimulate or inhibit toxin production, depending on the bacteria species and toxin type. S. haemolyticus from the museum produced lipase and deoxyribonuclease. It was found that Corynebacterium lubricantis and Candida parapsilosis did not produce any of the tested virulence factors
Conclusions:
In the work environment in composting, tanneries, museums with high frequency (56–100%) there are potentially pathogenic organisms: Bacillus cereus, B. pumilus, B. subtilis, Cryptococcus albidus, Pseudomonas vancouverensis, Staphylococcus heamolyticus able to produce virulence factors (polysaccharide capsules, proteinase, gelatinase, lipase, coagulate, deoxyribonuclease, enterotoxins, haemolysins).
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| eISSN: | 2084-6312 |
| ISSN: | 1505-7054 |
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