RESEARCH PAPER
The combined effect of scutellaria baicalensis extract and coenzyme Q10 in oxidative stress induced by chromium compounds
1
Katedra i Zakład Toksykologii Akademii Medycznej we Wrocławiu
Kierownik Katedry Toksykologii: prof. dr hab. Anna Długosz
Med Srod. 2010;13(1):72-77
KEYWORDS
ABSTRACT
Background:
The common use of antioxidants and its joint application brings the question whether they are useful in oxidative stress induced by the chemicals or whether they cause harmful interaction. Both Scutellaria baicalensis and CoQ10 are known as antioxidants, however one exogenous, the second endogenous. Chromium belongs equal to essential microelements and toxic factors. Therefore the aim of work was the evaluation joint effect of two examined antioxidants in exposure to chromium compounds.
Material and Methods:
The material was fresh blood obtained from healthy volunteers. The concentration of malondialdehyde (MDA) in erythrocytes was evaluated using Stock’s method. The activity of mixture of Antoxyd and coenzyme Q10 was tested after exposure to chromium III and VI at concentrations: 0,05; 0,5 and 1,0 μg/ml. Antioxidants were used in concentrations : 8,0; 20; 60 and 100 μg/ml.
Results:
The influence of coenzyme Q10 in exposure to chromium III and chromium VI was statistically insignificant, but CoQ10 given together with Antoxyd in all used concentration statistically significant decreased the level of MDA in erythrocytes exposed to chromium compounds (p<0,001).
Conclusions:
Application of both antioxidants has exerted synergistic action lowering MDA level, which was elevated after chromium. No harmful interactions in the examined sample between antioxidants and chromium ions were noted.
The common use of antioxidants and its joint application brings the question whether they are useful in oxidative stress induced by the chemicals or whether they cause harmful interaction. Both Scutellaria baicalensis and CoQ10 are known as antioxidants, however one exogenous, the second endogenous. Chromium belongs equal to essential microelements and toxic factors. Therefore the aim of work was the evaluation joint effect of two examined antioxidants in exposure to chromium compounds.
Material and Methods:
The material was fresh blood obtained from healthy volunteers. The concentration of malondialdehyde (MDA) in erythrocytes was evaluated using Stock’s method. The activity of mixture of Antoxyd and coenzyme Q10 was tested after exposure to chromium III and VI at concentrations: 0,05; 0,5 and 1,0 μg/ml. Antioxidants were used in concentrations : 8,0; 20; 60 and 100 μg/ml.
Results:
The influence of coenzyme Q10 in exposure to chromium III and chromium VI was statistically insignificant, but CoQ10 given together with Antoxyd in all used concentration statistically significant decreased the level of MDA in erythrocytes exposed to chromium compounds (p<0,001).
Conclusions:
Application of both antioxidants has exerted synergistic action lowering MDA level, which was elevated after chromium. No harmful interactions in the examined sample between antioxidants and chromium ions were noted.
REFERENCES (20)
1.
Bagchi D., Bagchi M., Stohs S.J.: Chromium (VI)-induced oxidative stress, apoptotic cell death and modulation of p53 tumor suppressor gene. Molecular and Cellular Biochemistry 2001; 222: 149-158.
3.
Goulart M., Batoreu M.C., Rodrigues A.S. I wsp..: Lipoperoxidation products and thiol antioxidants in chromium exposed workers. Mutagenesis 2005; 20: 311-315.
4.
Vandana S., Ram S., Ilavazhagan M. I wsp. Comparative cytoprotective activity of vitamin C, E and beta-carotene against chromium induced oxidative stress in murine macrophages. Biomed Pharmacother 2006; 6: 71-76.
5.
Biedermann K.A., Landolph J.R.: Role of valence state and solubility of chromium compounds on induction of cytotoxicity, mutagenesis and anchorage independence in diploid human fibroblasts. Cancer Res 1990; 50: 7835-7842.
6.
Speetjens J.K., Collins R.A, Vincent J.B. i wsp.: The nutritional supplement chromium (III) tris(picolinate) cleaves DNA. Chem Res Toxicol 1999, 12:483-487.
7.
Sawicka E., Średnicka D., DΠugosz A.: Scutellaria baicalensis inhibits lipid peroxidation caused by chromium in human erythrocytes. Adv Clin Exp Med 2008; 17: 539-544.
8.
Sawicka E., DΠugosz A., Roszkowska A.: Synergistyczne działanie wyciągu z tarczycy bajkalskiej i koenzymu Q10. Brom Chemia Toksykol 2009, 1.
9.
Bentinger M, Brismar K, Dallner G.: The antioxidant role of coenzyme Q. Mitochondrion 2007; 7(Suppl 1): S41–50.
10.
Cheng PY., Lee YM., Wu YS. i wsp.: Protective effect of baicalein against endotoxic shock in rats in vivo and in vitro. Biochem Pharmacol 2007; 73: 793-804.
11.
Gawlik M: Zastosowanie modelu krwinki czerwonej człowieka do badania właściwości antyoksydacyjnych związków zawartych w wodnych ekstraktach produktów spożywczych na przykładzie herbaty. Brom Chem Toksykol 2004, XXXVII, 4, 311-316.
12.
Stocks J, Offerman EL: Model c.b., Dormandy t.L.; the susceptibility to autooxidation of human red cell lipids in health and disease. Br J Haematol 1972, 23, 713-724.
13.
Wataha JC., Lewis JB., Lockwood PE. i wsp. Effect of dental metal ion on glutatione levels In THP-1 human monocytes. J Oral Rehabil 2000; 27: 508-516.
14.
Jun F., Xiang L., Yue C. i wsp.: Oxidative stress as a component of chromium-induced cytotoxicity in rat calvarial osteoblasts. Cell Biol Toxicol 2008: 24:201-212.
15.
Kalahasthi RB, Raghavendra RRH, Krihna MRB, Karuna KM: Effect of chromium (VI) on the status of plasma lipid peroxidation and erythrocyte antioxidant enzymes in chromium plating workers. Chem Biol Interact 2006; 164, 3: 192–199.
16.
Messer RLW., Doeller JE, Kraus DW. i wsp.: An investigation of fibroblast mitochondria enzyme activity and respiration in response to metal ions released from dental alloys. J Biomed Mater Res 2000; 50: 598-604.
17.
Długosz A., Sawicka E., Marchewka Z.: Styrene and ethylene glycol have a synergetic effect on lipid peroxidation that is better protected than repaired by CoQ10. Toxicol in Vitro 2005;19:581–588.
18.
Ueng Y.F, Shyu CC, Liu TY I wsp.: Protective effect of baicalein and wogonin against benzo[a]pyrene and aflatoxin B(1)– induced genotoxicitie. Biochem Pharmacol 2001; 62: 1653–1660.
19.
Gao Z, Huang K, Xu H: Protective effect of flavonoids in the roots of Scutellaria baicalensis Georgi against hydrogen peroxide- induces oxidative stress in HS-SY5Y cells. Pharmacol Res 2001; 43: 173-179.
20.
Kalahasthi RB., Raghavendra RRH., Krihna MRB. i wsp.: Effect of chromium (VI) on the status of plasma lipid peroxidation and erythrocyte antioxidant enzymes in chromium plating workers. Chem Biol Interact 2006, 164, 3, 192–199.
Share
RELATED ARTICLE
| eISSN: | 2084-6312 |
| ISSN: | 1505-7054 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
