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RESEARCH PAPER
Fungal aerosol in public utility buildings in the city of Kraków
 
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Department of Microbiology, Faculty of Agriculture and Economics, University of Agriculture in Kraków, Poland. Head of the Department of Microbiology: Assoc. Prof. PhD Eng. M.J. Chmiel Rector of the University of Agriculture: Prof. W. Sady
 
 
Corresponding author
Anna Lenart-Boroń   

Department of Microbiology University of Agriculture in Kraków Mickiewicza ave 24/28 phone no.: +48126624095
 
 
Med Srod. 2015;18(1):18-24
 
KEYWORDS
ABSTRACT
Introduction:
The quality of indoor air is one of the most important factors affecting health and well-being of people, who inhale 10m3 of air every day and spend 80–95% of life indoors. The aim of this research was to evaluate the occurrence of airborne fungi, along with assessment of air pollution and microbiological hazard for humans, in 45 public utility buildings in Cracow.

Material and Methods:
The study was carried out in four groups of buildings, including teaching facilities of the University of Agriculture and Jagiellonian University, churches, shopping malls and hospitals with outpatient clinics. Four sites located in the open air were chosen as control. The air sampling was carried out with MAS-100 impactor. Fungi were enumerated on Malt Extract Agar and the results were expressed as colony forming units (CFU) per m3 of air. The isolated fungi were identified by comparing macroscopic and microscopic observations with taxonomic monographs.

Results:
Mean concentration of airborne fungi was highest in the teaching facilities (1970 CFU/m3). These were also the sites where the largest range of fungal concentration was observed, i.e. from 0 to 23,300 CFU/m 3 . The lowest mean concentration (99 CFU/m3) including range was observed in hospitals (from 0 to 327 CFU/m3). Species identification of the fungal isolates revealed the presence of allergenic fungi (Alternaria and Cladosporium) in the examined spaces. Moreover, some strains were also identified as potentially toxigenic species, such as Penicillium expansum or Aspergillus niger.

Conclusions:
The concentration range of airborne fungi varied significantly between the tested spaces. Although the observed concentration of airborne fungi in the majority of buildings was quite low, detection of potentially toxigenic fungi indicates the need for monitoring of both concentration and composition of fungal aerosol in public utility buildings.

FUNDING
this study was financed by the statutory measures of the Department of Microbiology, University of Agriculture in Kraków.
 
REFERENCES (23)
1.
Dacarro C., Picco A.M., Grisoli R. et al.: Determination of aerial microbiological contamination in scholastic sports environment. J Appl Microbiol. 2003; 95: 904-912.
 
2.
Kalwasińska A., Burkowska A., Wilk I.: Microbial air contamination in indoor environment of a university library. Ann Agric Environ Med. 2012; 19: 25-29.
 
3.
Newson R., Strachan D., Corden J. et al.: Fungal and other spore counts as predictors of admission for asthma in the Trent region. Occup Environ Med. 2000; 57: 786-792.
 
4.
Renn P., Jankun T.M., Belanger K. et al.: The relation between fungal propagules in indoor air and home characteristics. Allergy 2001; 56: 419-424.
 
5.
Allsopp D., Seal K.J., Gaylarde C.C.: Introduction to biodeterioration. 2nd Ed. Cambridge University Press, Cambridge 2004: 252.
 
6.
Ayanbimpe G.M., Danjuma S., Okolo M.O.: Relationship between fungal contamination of indoor air and health problems of some residents in Jos (in:) Kumar S. (ed.): Air quality– monitoring and modeling. Intech Europe, Rijeka 2012: 1-19.
 
7.
Annesi-Maesano I.: Indoor exposition to molds and health outcome. Review of Allergy and Clinical Immunology 2013;23: 21-25.
 
8.
Shelton B.G., Kirkland K.H., Flanders W.D. et al.: Profiles of airborne fungi in buildings and outdoor environments in the United States. Appl Environ Microbiol. 2002; 68: 1743-1753.
 
9.
Operator’s Manual MAS-100TM professional Microbial Air Monitoring System for the Microbiological Testing of Air.Brussels, Belgium.
 
10.
Fassatiova O.: Microscopic fungi in technical microbiology. Scientific and Technical Publishing. Warsaw, 1983. [in Polish].
 
11.
Samson R., Frisvad J.: Penicillium subgenus Penicillium, new taxonomic schemes, mycotoxins and other extrolites. Stud Mycol. 2004; 49: 1-251.
 
12.
Gołofit-Szymczak M., Górny R.L.: Bacterial and fungal aerosols in air-conditioned office buildings in Warsaw, Poland –the winter season. Int J Occup Saf Ergon. 2010; 16: 465-476.
 
13.
Augustyńska D., Pośniak M.: Harmful agents in the working environment – admissible values. CIOP-PIB. Warsaw, 2007.
 
14.
Cox C.S., Waters C.M.: Bioaerosols handbook. Lewis Publishers, New York, 2005.
 
15.
Lis D.O., Mainelis G., Górny R.L.: Microbial air contamination in farmhouses – quantitative aspects. Clean – Soil, Air,Water 2008, 36: 551-555.
 
16.
Hsu Y.C., Kung P.Y., Wu T.N. et al.: Characterization of indoor-air bioaerosols in southern Taiwan. Aerosol Air Qual Res. 2012, 12: 651-661.
 
17.
Polish Standardization Committee. PN-Z-04111-03. Protection of air purity – Microbiological tests – Determination of the number of microscopic fungi in ambient air (immission) when sampling with aspiration and sedimentation methods. Warsaw, 1989. [in Polish].
 
18.
Lugauskas A., Krikštaponis A.: Microscopic fungi found in libraries of Vilnius and factors affecting their development. Indoor Built Environ. 2004, 13: 169-182.
 
19.
Dutkiewicz J., Górny R.L.: Biological factors hazardous to human health: classification and criteria of exposure assessment. Occup Med. 2002, 53: 29-39. [in Polish].
 
20.
Polish Minister of Health. Journal of Laws of the Republic of Poland No. 05.81.716 Ordinance of the Minister of Health from April 22nd 2005 on hazardous biological agents in the workplace and on the health protection of workers occupationally exposed to these agents.
 
21.
Gomez de Ana S., Torrez-Rodrigues J.M., Alvarado-Ramirez E. et al.: Seasonal distribution of Alternaria, Aspergillus, Cladosporium and Penicillium species isolated in homes of fungal allergic patients. J Investig Allergol Clinical Immunol. 2007, 16: 357-363.
 
22.
Menteşe S., Arisoy M., Rad A.R. et al.: Bacteria and fungi levels in various indoor and outdoor environments in Ankara, Turkey. Clean – Soil, Air, Water 2009, 37: 487-493.
 
23.
Pastuszka J.S., Tha Paw U.K., Lis, D.O. et al.: Bacterial and fungal aerosol in indoor environment in Upper Silesia, Poland. Atmos Environ. 2000, 34: 3833-3842.
 
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