RESEARCH PAPER
Beneficial effect of selenium and magnesium on reduction of oxidative stress in brain of rats intoxicated with alcohol
1
Katedra i Zakład Higieny, Akademia Medyczna im. Piastów Śląskich we Wrocławiu
Kierownik Katedry – prof. dr hab. Krystyna Pawlas
Corresponding author
Iwona Markiewicz-Górka
Katedra i Zakład Higieny Akademii Medycznej im. Piastów Śląskich we Wrocławiu ul. J. Mikulicza-Radeckiego 7 50-368 Wrocław tel. +48 71 7841505, fax +48 71 7841503
Katedra i Zakład Higieny Akademii Medycznej im. Piastów Śląskich we Wrocławiu ul. J. Mikulicza-Radeckiego 7 50-368 Wrocław tel. +48 71 7841505, fax +48 71 7841503
Med Srod. 2012;15(2)
KEYWORDS
ABSTRACT
Introduction:
The aim of the study was to estimate the influence of supplementation with selenium and/or with magnesium the activities of antioxidative enzymes and alleviation of oxidative stress induced by ethanol in the rats brains. Forty male rats of Wistar strain were organized into five groups: 1 control and 4 groups which were given to drink alcohol. Rats were intoxicated with 15% solution of ethanol 4 days a week, and tap water and also water supplemented with selenium (0.4 mg Se/l), magnesium (100 mg Mg/l) or simultaneouslywith magnesium and selenium for the next 3 days. The experiment has been carried out for over 3 months. The intoxication of rats with ethanol decreased catalase activity (about 20%) and significantly (about twice) rose the concentration of malonylodialdehyde (MDA) in brains in comparison with the control animals. Magnesium and/or selenium administration increased catalase and superoxide dismutase activities and lowered concentration of MDA in brains. In rats drinking alcohol increase of activity of glutathione peroxidise (GPx) was noted. The highest glutathione peroxidase and catalase activities were found in brains of rats supplemented with magnesium and selenium simultaneously
Conclusions:
Increased supply of Se and/or Mg during the periods of abstinence increases the activities of enzymes neutralizing free radicals and lessens oxidative alcohol induced stress in brains of rats.
The aim of the study was to estimate the influence of supplementation with selenium and/or with magnesium the activities of antioxidative enzymes and alleviation of oxidative stress induced by ethanol in the rats brains. Forty male rats of Wistar strain were organized into five groups: 1 control and 4 groups which were given to drink alcohol. Rats were intoxicated with 15% solution of ethanol 4 days a week, and tap water and also water supplemented with selenium (0.4 mg Se/l), magnesium (100 mg Mg/l) or simultaneouslywith magnesium and selenium for the next 3 days. The experiment has been carried out for over 3 months. The intoxication of rats with ethanol decreased catalase activity (about 20%) and significantly (about twice) rose the concentration of malonylodialdehyde (MDA) in brains in comparison with the control animals. Magnesium and/or selenium administration increased catalase and superoxide dismutase activities and lowered concentration of MDA in brains. In rats drinking alcohol increase of activity of glutathione peroxidise (GPx) was noted. The highest glutathione peroxidase and catalase activities were found in brains of rats supplemented with magnesium and selenium simultaneously
Conclusions:
Increased supply of Se and/or Mg during the periods of abstinence increases the activities of enzymes neutralizing free radicals and lessens oxidative alcohol induced stress in brains of rats.
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